Welcome to stPlus, a reference-based method for the accurate enhancement of spatial transcriptomics

Based on an auto-encoder with a carefully tailored loss function, stPlus performs joint embedding and predicts spatial gene expression via a weighted k-NN.

• stPlus outperforms baseline methods in accurately predicting gene expression.
• The spatial transcriptomics enhanced by stPlus can provide better identification of cell populations.
• stPlus is robust and scalable to datasets of diverse gene detection sensitivity levels, sample sizes, and number of spatially measured genes.