To investigate the difference between validated silencers and other open regions, we systematically compared validated silencers and other open regions in human K562 cell line. We obtained two datasets of open regions in K562 (DNase-seq peaks and ATAC-seq peaks) from NCBI as a baseline. The length distributions of validated silencers and that of open regions in K562 are shown in Figure 2A. The lengths of silencers are mainly between 100~300bp, while the lengths of open regions are mainly between 200~400 bp for the DNase-seq dataset and 600~1000 bp for the ATAC-seq dataset (Figure 2A). The distributions of distance from silencers and open regions to corresponding nearest genes are shown in Figure 2B. The average distance from a silencer to its nearest coding gene is 61,721 bp, which is significantly less than that from a DNase-seq peak to its nearest coding gene (144,767 bp, one-sided Wilcox test p-value<2.2e-16)) and from an ATAC-seq peak to its nearest coding gene (132,892 bp, one-sided Wilcox test p-value<2.2e-16)). The GC content distribution of regions in different datasets is shown in Figure 2C. On average, the GC content of validated K562 silencers is 0.4896, which is much higher than that of DNase-seq peaks (0.4266, one-sided Wilcox test p-value<2.2e-16) and ATAC-seq peaks (0.4222, one-sided Wilcox test p-value<2.2e-16). We performed one-sided Wilcoxon test and two-sided Wilcoxon test for silencer openness scores in K562 against the remaining 198 cell lines and obtained 198 FDR p-values using Benjamini and Hochberg correction respectively. The one-sided and two-sided p-value distributions are shown in Figure 2D, suggesting the cell line specificity of silencers.